Journal:

Article Title: Gene expression profiling in autoimmune non-infectious uveitis disease

doi:

Figure Lengend Snippet: Scatter plot analysis of gene expression profiling on uveitis patients demonstrates differential gene expression. cDNA microarray analysis on 50 RNA samples from uveitis patients and 1 pooled sample from controls was performed. After normalization on house-keeping genes, e.g, GAPDH and beta-2-microglobulin (B2M), the final scores of each of the genes of all arrays were compared to those from the control array. The scatter plot was acquired as described in the Materials and Methods. The Y-axis represents log scores from uveitis patients (group 2) and the X-axis represents log scores from normal controls (group 1). Each symbol represents one gene with red-colored ones represent 2-fold higher expressed in uveitis patients (group 2), green-colored ones represent 2-fold lower expressed in uveitis patients and the black-colored ones represent within 2-fold cut-off threshold.

Article Snippet: Probes were hybridized to a pathway-specific cDNA microarray chip (Inflammatory and autoimmune GEArray; SuperArray Inc., Gaithersburg, MD).

Techniques: Gene Expression, Microarray, Control

Journal:

Article Title: Gene expression profiling in autoimmune non-infectious uveitis disease

doi:

Figure Lengend Snippet: Clustergram analysis of gene expression revealed 4 distinct gene expression profiles. cDNA microarray was performed as described above and clustergram analysis on microarray data was described in the Materials & Methods section. X-axis represents all genes listed in the cDNA microarray chip and Y-axis depicts gene expression profiles from all samples of uveitis patients normalized on normal controls. Each colored band represents expression of a single gene from one patient when compared to that from normal donors with higher expression in red and lower expression in green. Groups I, II, III and IV designate 4 sub-groups of gene expression profiles. Analysis of gene expression profiling among uveitis patients revealed 4 sets of genes designated as molecular signatures A, B, C and D that can be used to define the 4 sub-groups of gene expression profiles.

Article Snippet: Probes were hybridized to a pathway-specific cDNA microarray chip (Inflammatory and autoimmune GEArray; SuperArray Inc., Gaithersburg, MD).

Techniques: Gene Expression, Microarray, Expressing

Journal:

Article Title: Gene expression profiling in autoimmune non-infectious uveitis disease

doi:

Figure Lengend Snippet: Comparison of clustergram analysis of gene expression profiling of 2 male siblings (designated as patient A and patient B) with the same diagnosis of intermediate uveitis with that from normal controls (designated as NL). The cDNA microarray and clustergram analysis were performed as described above and in the Materials and Methods. The X-axis listed all the gene symbols in the cDNA microarray chips. The gene expression profiles from the 2 male siblings with the same diagnosis of intermediate uveitis (designated as patient A and patient B) were aligned with the profile from normal subjects (NL). As described above, each colored band represents expression of a single gene from either patient A or patient B or from normal subjects after normalization on house keeping genes, with higher expression in red and lower expression in green.

Article Snippet: Probes were hybridized to a pathway-specific cDNA microarray chip (Inflammatory and autoimmune GEArray; SuperArray Inc., Gaithersburg, MD).

Techniques: Comparison, Gene Expression, Biomarker Discovery, Microarray, Expressing